Lab 2 HISTOLOGY
I. Histology - Make
sure that you are able to do the following:
1. Identify
the slides and the structures listed.
2. Know
where these tissues are found in the body.
3. Become
very familiar with how these slides look under
different powers.
Epithelial Tissue Drawing of Tissue
1. Squamous Epithelium
Note the shape of these isolated cells.
2. Stratified Squamous-Skin
epidermis
3. Simple Squamous-Kidney
nucleus
4. Simple Cuboidal-Thyroid
nucleus
basement membrane
connective tissue
5. Simple Columnar
goblet cell
nucleus
basement membrane
connective tissue
6. Transitional
nucleus
basement membrane
connective tissue
7. Pseudostratified Ciliated Columnar Epithelium
cilia
goblet cell
nucleus
basement membrane
connective tissue
Connective Tissue
1. Areolar
elastic fibers
collagenous fibers
fibroblast
2. Adipose (modification of areolar)
nucleus
fat globules
3. Collagenous/Dense Fiberous
fibroblasts
bundles of collagen
4. Elastic tissue
fibroblast
elastic fibers
5. Hyaline
Cartilage
perichondrium
lacuna
chondrocyte
6. Elastic Cartilage
lacuna
chondrocyte
perichondrium
7. Fibrocartilage
lacuna
condrocyte
8. Blood
Identify an erythrocyte (RBC) and a leukocyte (WBC)
Muscle Tissue
1. Smooth
nucleus
muscle fiber (cell)
2. Cardiac
nucleus
intercalated disc
striations
muscle fiber (cell)
3. Skeletal
nucleus
muscle fiber (cell)
striations
Nerve Tissue
1. Giant Multipolar Neuron
cell body
dendrites
axon
nucleus
2. Medulated (mylenated) Nerve
Node of Ranvier'
myelin sheath
axon
nucleus of Schwann cell
3. Neuroglia
astrocyte
II. Skeletal material - none
III. Models
A. Cardiac Muscle; note the following:
1. Purkinje cell, showing superficial arrangement of fibrils in B and C
2. Nucleus of Purkinje cell
3. Sarcoplasm (abundant in Purkinje cells, scant in heart muscle cells)
4. Myofibrils (evenly distributed in heart muscle cells, peripheral in Purkinje cells)
5. Nucleus of heart muscle cell
6. Intercalated disks
7. Sarcolemma
8. Area of heart muscle cell with sacrolemma removed to show myofibrils
9. Sympathetic nerve fiber
10. Nerve ending on myofibrils
11. Connective tissue, envelops all fibers and serves as a matrix for the nerves and blood vessels.
12. Nucleus of a connective tissue cell
13. Longitudinal vessel of capillary network
14. Capillary loop
B. Skeletal Muscle - Note the following:
1. Notched ending of the type commonly arising from periosteum
2. Rounded ending of the type usually seen in union with a tendon
3. Tapered ending, characteristic of terminations within the body of the muscle
4. Cross-section of a fiber, showing peripheral nuclei and myofibrils grouped in Cohnheim's fields
5. Cross-section of fiber with peripheral and central nuclei and myofibrils arranged in Cohnheim's fields
6. Cross-section of fiber showing myofibrils uniformly distributed throughout sarcoplasm
7. Sarcolemma
8. Central nucleus
9. Sarcolemma removed to show the myofibrils
10. Peripheral nucleus
11. Peripheral nucleus underlying sarcolemma
12. Connective tissue (endomysium). This ensheaths all fibers of the muscle and serves as a pathway for nerves
and blood vessels.
13. Nucleus of a connective tissue cell
14. Myelinated nerve fiber
15. Non-myelinated nerve fiber
16. Motor end-plate underlying sarcolemma (hill of Doyere)
17. Sarcolemma and sarcoplasm removed to expose motor end-plate
18. Capillary net. Skeletal muscle is highly vascular
C. Myofibril Model - Note the Following:
1. Z Line
2. I Band
3. A Band
4. H Zone
5. M Line
6. Terminal Cysternae
7. Transverse Tubule (T-Tubule)
8. Longitudinal Tubules
9. Ribosomes
10. Mitochondria
11. Sarcolemma
12. Opening of T-Tubule
D. Sarcoplasmic Reticulum, Skeletal
1. Z-line
2. I-band
3. A-band
4. H-zone
5. M-line
6. Myosin fibers (M-band)
7. Actin fibers (I-band)
8. Actin & Myosin fibers (A-band)
Sarcoplasmic reticulum (SR)
9. Longitudinal tubules
10. Terminal Cysternae
11. M-rete of SR
12. Z-rete of SR
13. Transverse tubule
14. Interior coupling
15. Peripheral coupling
16. Transverse tubule opening
17. Sarcolemma
18. Sarcoplasm
19. Mitochondrion
20. Glycogen granules
E. Visceral Muscle - Note the following:
1. Capillary - The blood supply of smooth muscle is scanty compared to
that of skeletal and cardiac muscle.
The prominence of the vessels on the model is due to the selection of a
location near a capillary junction to show the manner of branching.
The capillaries appear larger in this than in the other two models because of the difference in magnification.
2. Connective tissue, seen here between the muscle cells. This consists of
elastic and collagenous fibers.
Its quantity varies greatly in different organs.
3. Connective tissue, seen here partly ensheathing a cell. The connective
tissue actually encloses all the muscle cells throughout their length,
binding them into a tissue.
4. Nucleus of a connective tissue cell. In some layers of smooth muscle
connective tissue cells are either lacking or difficult to demonstrate.
5. Motor end-plate of autonomic nerve fiber.
6. Smooth muscle cell, cut away longitudinally to show myofibrils.
A sarcolemma is lacking.
7. Nucleus of a smooth muscle cell
8. The same in tranverse section
F. The Neuron
A. The cell body
B. Peripheral nerve with associated cells
1. Axon hillock
2. Nucleus
with nucleolus
3. endoplasmic reticulum (Nissl's granules)
5. bouton
6. axon
7. Schwann Cell with nucleus
8. myelin sheath
9 Node of Ranvier'
10. mitochondria
11. layers of myelin
12. connective tissue
13. cross section of axis cylinder
14. dendrite
15. lysosomes
16. neurotubules
17. Golgi apparatus
VI. Dissection - None