by James Bolton
Abstract
In the freswater single celled alga Chlamydomonas reinhartii, extra cytosolic water, gained osmotically, is separated and deported to the cell exterior by the contracting of the contractile vacuole complex. A liquid culture of Chlamydomonas was given a dosage of UV light to cause gene mutations that affect the organism's contractile vacuole. Mutations assist in explaining the processes that contribute to the contractile vacuole cycle in Chlamydomonas. From over 2,000 irradiated colonies that were isolated, six show signs of normal growth but poor motility in low osmotic strength, and one of the six showed poor growth in low osmotic strength.
by Jonnica Dozier
Abstract
The objective of this experiment was to create a hemicacerand with TTF walls to determine if the redox properties of TTF will affect the guest once it enters the hemicarcerand. Our goal was to determine if the properties of the guest can be controlled by the host. The procedure leading to the formation of of a hemicarcerand with tetrathiafulvalene walls is outlined and yeilds are given.
by Romni Owens
Abstract
The purpose of this experiment was to synthesize small versions of a natural enediyne and see which one had the lowest activation energy for Bergman cyclization. Electron deficient enediynes are believed to react faster than electron rich ones, and may be important in the synthesis of new antibiotics. Our attempt to synthesize enediynes was ultimately unsuccesful.
by Luisel Santi-Ruiz
Abstract
The few data avaialable on the reliance of anural larvae on anaerobic metabolism indicate that glycolytic is signifigantly less than that of adults. The transition betwenn larval and adult glycolytic capacity has not been fully investigated. The effects of age on resting and active lactate concentrations of larval Osteopilus septentrionalis was measured in the present study. Lactate concentrations and glycolytic capacties did vary with age. This may have been cause by morphological and biochemical changes during development.
by Sherika Spear
Abstract
The origin of ligand binding in hemeproteins such as myoglobin (Mb) and legheamoglobin (LeMb) was investigated by evaluating molecular dynamics simulations starting from x-ray crystallographic structures determined at 1.8 Angstrom resolution. The nature of intermolecular interactions and intermolecular effects that modulate the Mb Carbon monoxide (CO) and Oxygen (O2) binding ratio has been a subject of intense debate.
by LaDexter D. Vinson
Abstract
Optimal foraging theory states that an organism will gather resources from a particular area if it feels that it is benefitting from its foraging efforts in that area. This theory explains and organism's decision to remain in one patch and forage or move to another patch. Optimal foraging can be measured by using the 'giving up densities (GUD) method. To examine optimal foraging, seed trays were used to test GUDs in a rodent community in Key Largo, Florida.
by Edward L. Aikens
Abstract
The current information concerning the targeting of proteins from the Golgi Apparatus to the lysosome is primarily explained using the mannose 6-phosphate (m6p) dependent system. This research project is interested in a m6p independent system of targeting, for the sorting of lysosomal enzymes from the Golgi network. A model protein associated in the m6p independent targeting of Cathepsin D, a lysosomal enzyme, has been identified and purified in small amounts from the Golgi of rat liver cells. This segment of the research is concerned with developing the most effective way of purifying a larger amount of this protein, in order to do amino acid sequencing, and subsequently learn more about this targeting system. Two-dimensional SDS-Polyacrylamide Gel Electrophoresis, a technique cited for its high level of sensitivity and resolution, is the method that was chosen to accomplish this task. It enables the isolation of proteins based upon two independent parameters(i.e. isoelectric point, molecular weight). The following variables were manipulated in order to optimize the amount of protein isolated: sample volume, sample load, sample preparation, and run time and settings. The experimental data resulting from these manipulations were not very conclusive. There was consistently a problem with insufficient protein isolation and streaking. Further experimentation is needed before two-dimensional Gel Electrophoresis can be used to isolate this particular protein.
by Oladapo A. Alade
by Kristin Dixon
Abstract
The ideal free distribution was proposed to predict how animals distribute themselves among habitats which may vary in patch quality in order to maximize their gain. In this paper, I tested male Gambusia holbrooki to see if they distributed themselves according to the ideal free distribution with respect to mating opportunities. I accomplished this by testing males with 3 different treatments of females: one in which no females were present, one in which females were presented in equal patches of 3:3, and one in which females were presented in unequal patches of 2:4. When no females were present, the observed mean number of males on any one side did not significantly differ from the expected value of 3. The observed mean number of males in the treatments with unequal patches differed significantly from means expected by the ideal free distribution. However, the observed distribution of males in the treatment with unequal patches of females differed significantly from those treatments in which females were not present or were in equal patches. All of the treatments did not match precisely the predictions of the ideal free model.
by LaVictoria Green
Abstract
The purpose of this paper was to determine possible patterns of root establishment of big and small trees in the soil profile of a tropical seasonal forest. This was done by using stable isotope techniques. A site located in Paragominas, Brazil was used to collect stem and root samples. As the diameter at breast height (dbh) of the trees increased, so did the d13C value. Briscoe and Wadsworth found (I970) a positive correlation between the d13C values of stems and roots of the same tree. As the soil depth increased, the variation of the d13C values of the roots increased. At the surface the range was from 27-29 0/00, while at four meters the range was from 27-32 0/00. It was concluded that root establishment of big and small trees in the soil is heterogeneous both vertically and horizontally.
by Craig Smith
Abstract
The paper presents the results of a study of habitat usage of two rodent species in Key Largo, Florida. Habitat usage was evaluated by multiple regression analysis. Vegetative variance was then be combined with capture data of P. g. allapaticola and N. f. smalli over a two year period. This analytical approach can lead to the determination of habitat use, and provide insights on how to better manage habitat resources. As in the case P. g. allapaticola and N. f. smalli, both of which are on the endangered species list.
by Jumoke K. K. Alim
by Cory L. Cain
by Donnie Eddins
by Sybil Fisher
By Lauren Jones
Abstract
New osmoregulatory mutants of Chlamydomonas reinhardtii are tested for the appearance of the same mutated genes as those expressed in the known mutants. Mutants defective at different stages of the contractile vacuole system of C reinhardtii are helpful in defining the steps of the process that will eventually lead to an explanation of how this process takes place.
The new mutants, osm 14, osm 15 and osm 16 have been proven to be osmoregulatory mutants. They contain different mutated genes from the known mutants and can be added to the list of osmoregulatory mutants. There were previously eight known mutants and with the addition of these three new mutants there are now eleven osmoregulatory mutants that control the contractile vacuole cycle.
by Lorraine Kombudo
by Kia M. Mitchell
by Alexis Tapanes
Abstract
Peromyscus gossypinus allapaticola were live-trapped on four grids in northern Key Largo. Homerange was analyzed at 95% by minimum convex polygon using the CALHOME program. The effects sex, number of times caught, time period trapped over, grid location, and density of mice had on homerange were examined. Males had a significantly larger homerange than females. The. homerange of males increased as the time period they were trapped over increased, and the correlation between male homerange size and number of times trapped approach significance. Female homerange size increased as the number of times females were trapped increased, but there was no correlation between female homerange and number of trapping sessions caught. Neither grid location nor density of cotton mice per grid affected homerange size.
by Sonya Renee’ Carter
by Myron Stokes
by Gregory O’niel Crawford
by Jibri Wiggins