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In order to completely
assess the effects of atrazine on the development of the reproductive organs
of these toads, I will have to
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1.Be able to describe the neuroanatomy of both the Oak Toad and the
Marine Toad
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2.I must be able to use special staining methods to identify sexually
different areas of the brain
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3.With this information, I will be able to pinpoint the timing of sexual
differentiation Ðthe time when the brain is distinctly male or femaleÑof the
brain of these two species. I
will also determine the timing of sexual differentiation of the gonads.
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4.Finally, I will treat animals with atrazine and asses the possible
effects on the sexual differentiation of both the brain and the gonads.
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