In order to completely assess the effects of atrazine on the development of the reproductive organs of these toads, I will have to
1.Be able to describe the neuroanatomy of both the Oak Toad and the Marine Toad
2.I must be able to use special staining methods to identify sexually different areas of the brain
3.With this information, I will be able to pinpoint the timing of sexual differentiation Ðthe time when the brain is distinctly male or femaleÑof the brain of these two species.  I will also determine the timing of sexual differentiation of the gonads.
4.Finally, I will treat animals with atrazine and asses the possible effects on the sexual differentiation of both the brain and the gonads.