Gas Exchange II

Gas Exchange and Transport - Carbon Dioxide and Oxygen

1. Oxygen transport in humans.

2. Invertebrate oxygen binding pigments

  • Hemoglobin is found in invertebrates but some invertebrates have other types of heme pigments, including hemerythrin, chlorocruorin, and hemocyanin. Some invertebrates have no respiratory pigment at all. Read the small paragraph on invertebrate respiratory pigments in your text, pg. 531. As an example of how some invertebrate respiratory pigments are used, note that the hemocyanin of Limulus, the horseshoe crab, consists of 48 subunits, not four as in hemoglobin. Hemocyanins and other invertebrate respiratory pigments dissolve directly in the blood, not in blood cells. Hemocyanins use two copper atoms to bind oxygen instead of one iron, as in each subunit of hemoglobin.

3. Carbon dioxide transport in humans.

  • Carbon dioxide reacts with water to form carbonic acid that then dissociates into H+ and bicarbonate. This reaction can be catalyzed by the enzyme carbonic anhydrase, that speeds up the conversion by as much as ten million times!!!! Carbonic anhydrase is found inside the red blood cells but not in the blood plasma. The linked figure shows the active site of carbonic anhydrase, with the zinc atom held by the histidine side chains of the protein. Note that hemoglobin uses histidines to hold the heme group containing an iron atom.

  • Carbon dioxide diffuses from tissues into the blood plasma and then into red blood cells. Some of the CO2 binds to amino groups on hemoglobin, but most is converted into bicarbonate. (Eckert, Fig. 13-10a) These reactions are reversed in the lungs (Eckert, Fig. 13-10b). QUESTION - Why are the reactions reversed in the lungs? We will see one part of the answer in conjunction with the Haldane effect. Can you think of another, even more basic reason?

  • Bicarbonate transport across the red blood cell membrane involves simultaneous transport of chloride in the opposite direction. This transport, by Band III protein channels, is known as the chloride shift

  • The Haldane effect: (Eckert, Fig. 13-11 - FIGURE CORRECTED AND MODIFIED)

Human Lung Disease

Today, we are seeing a very young patient who is having chronic difficulty in breathing. Since the patient is in great distress, we will try to make a rapid diagnosis.

  • Our first guess is that the patient has asthma. This disease has three main features: 1) inflammation of the airway wall, 2) obstruction of the lumen of the airways by mucus, and 3) vasodilation of the bronchial blood vessels, causing increased vascular permeability and edema - water buildup. The inhaled allergen binds to IgE immunoglobulin on Mast cells that then release histamines, leucotrienes and other chemicals. The drug Singular blocks leukotriene receptors instead of blocking the histamine pathway as some older drugs do. (Diagram of bronchial asthma). Eosinophils, a type of white blood cell, are stimulated to release enzymes, including peroxidase, RNAse, DNAse, and lipases. Eosinophils normally help fight viral infections and parasites. In asthma, the inhaled allergen appears as a foreign invader and the eosinophils are recruited to fight the invasion. Alas, our patient doesn't seem to have asthma and we must look further ....

  • Perhaps our patient is suffering from emphysema. Our young patient is unlikely to be a long-term smoker. But, suppose we do have a smoker as a patient or the patient has been subject to some long term insult to the lung - working in a factory or a printing shop, for example. Smoking and other chronic insult to the lung increases the number of macrophages, phagocytotic, amoeba-like cells, perhaps in the alveoli or the bronchi. The macrophages respond by secreting chemoattractants for neutrophils. Neutrophils accumulate and release elastase. Elastase is an enzyme that breaks down the elastic fibers surrounding the alveoli and other parts of the lung. Another enzyme, called antitrypsin, breaks down the elastase and prevents it from destroying the alveolar wall. If the smoking persists, the antitrypsin is no longer produced in sufficient concentrations, levels of antitrypsin decrease, and tissue wall destruction follows. (Diagram of emphysema). Fortunately, our patient doesn't have emphysema.

  • The extreme youth of our patient, who turns out to be a baby, gives a clue - Epithelial cells lining the airways have CFTR ion pumps that use ATP to pump chloride out of the cells. Water flows out by osmosis, into the mucus secreted on the outside of the cells. Individuals with cystic fibrosis don't have working CFTR pumps. So, water no longer can follow the chloride movement, the mucus thickens and trapped bacteria lead to destruction of the epithelial cells. (Diagram of cystic fibrosis). Unfortunately, this is a difficult condition to treat as it is a genetic disease. One of the most promising treatment options may involve gene therapy.

Some of the material in this lecture follows the treatment of "Histology and Cell Biology" by Abraham L. Kierszenbaum (Mosby Press). I recommend it

All text and images, not attributed to others, including course examinations and sample questions, are Copyright, 2011, Thomas J. Herbert and may not be used for any commercial purpose without the express written permission of Thomas J. Herbert.